How to Run Positive and Negative Autotune

Topic

How to Perform Positive and Negative Autotune on an ISQ EC or ISQ EM

Environment

• ISQ EC Mass Spectrometer
• ISQ EM Mass Spectrometer
• Chromeleon software
• HESI source
• Positive and Negative ESI tuning

Steps/Procedure

1. Complete the calibrant delivery and tuning preparation procedures.

2. From the detector ePanel, click Tune.

3. Locate the Positive and Negative Autotune controls.

Run Positive Autotune

4. Click ESI Positive Tune.

5. Monitor the tuning status and progress displayed on the tuning page.

6. Allow Positive Autotune to complete.

7. Open the generated positive tune report.

8. Confirm that the tune passed.

Run Negative Autotune

9. Return to the Tune screen.

10. Click ESI Negative Tune.

11. Monitor the displayed tuning progress.

12. Allow Negative Autotune to complete.

13. Open the generated negative tune report.

14. Confirm that the tune passed.

Review Tune Results

15. Locate Available Tune Results on the Tune screen.

16. Review the reports identified by their date and time.

17. Identify the positive tune report by the PESI designation.

18. Identify the negative tune report by the NESI designation.

19. Confirm that both tuning programs passed before operating the detector.

Additional Information

• Both Positive and Negative Autotune must be performed even when the application uses only one polarity.
• Always run Positive Autotune before Negative Autotune.
• The training specifies retuning before and after repairs, before and after optics cleaning, or when known masses shift in the spectrum.
• If either tuning program fails, use ESI Diagnostic-Only Tune to begin troubleshooting.
• Additional troubleshooting information is available in the ISQ EC/EM Operator Manual and the ISQ EC and ISQ EM Single Quadrupole Mass Spectrometer: Routine Diagnostics and Troubleshooting training.