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Thermo Fisher Scientific

Eagle camera intensity difference in one quadrant

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Issue

Eagle camera intensity is different in one quadrant, compared to other three quadrants.

Environment

Tecnai Spirit

Resolution

These are possible resolutions for the issue:

  • Perform gain reference calibration.

Cause

These are possible causes for the issue:

  • Most likely gain offset change in Eagle camera electronics.

Details

After performing gain reference calibration, all four quadrants of the camera respond equally again.

If the problem re-occurs, first follow troubleshooting flow diagram in 4022 190 53708 (Troubleshooting diagram for Eagle quadrant problem).

In the end, if the problem cannot be solved by re-calibrating, it is possible that there is a defect on the analog board in the camera (the analog board has separate electronics for each of the quadrants). In that case, the boards should be replaced. Note: the boards (analog board, digital board and clock driver board) are linked as a set, so the boards need to be replaced together.

 

Problem situation:
clipboard_e65b3e8cef84cd4173b77b8b81a579752.png

 

After performing gain reference calibration:
clipboard_e61b4d6a690b81b5a1b1b80ecb028655e.png