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Thermo Fisher Scientific

Tomography v.5.2.0 - Tilt Series showing Stage Jumps~Shifts from Image to Image

Issue

Tomography v.5.2.0 – Tilt-Series showing Stage Jumps~Shifts from Image to Image.

Environment

Krios G3i (3.1)

Resolution

These are possible resolutions for the issue:

  • From the “Corrections” Section at Title-Bar for parameter settings of “Tracking – Switch Angle(°)” Option. 
    • Change from 10 degrees to 80 degrees.

Cause

These are possible causes for the issue:

  • Switch angle setting too small.

Details

How to Modify “Tracking – Switch Angle(°)”: 

  1. Go to the “Corrections” Section at Title-Bar. 
    • At the input Box of “Switch Angle” must be Gray-out unable to access. 
    • Gray-out to be protection for sudden modification. 
  2. Tick the Checkbox of “Track Before Acquisition”. 
    • This will Free-out the Access for new inputs. 
  3. Now to modify these “Switch Angle”.
    • Change from 10 Degrees to 80 Degrees
    • Enable for Stage to Actively perform Tilt-Series Corrections up to 80 Degrees.

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