Skip to main content
Thermo Fisher Scientific

Nicolet iS50 Aperture Size and Sample Image Size (Beam Diameter)

Issue

What is the image size (IR beam diameter) for a specific aperture size of the Nicolet iS50?

Environment

Nicolet iS50

Resolution

The table below presents the sample focus size (beam diameter) for the minimum to maximum aperture percentages.

Aperture % Aperture diameter (mm) Sample focus diameter (mm)
6.25% (min) 1.75 1.97
10% 2.21 2.5
20% 3.13 3.54
50% 4.95 5.59
100% 7 7.91
150% 8.57 9.69
230% (max) 10.62 11.99

Analysis

When totally closed, the aperture still has an hole of 1.75 mm in diameter.

Per optical definition, 7.0 mm in diameter of iris is 100%. By ratio, a 1.75 mm diameter hole is 6.25%, or 1.75/7.0.

By trial and error, it was determined that 7 mm iris amount to step count of 1007. This count shall linearly map to the square root of aperture setting (square root of area of the iris) from 100% to 6.25%, i.e.,

Step count = 1007 * [sqrt(n%) - sqrt(6.25%)] / [sqrt(100%) - sqrt(6.25%)]

Step count = 1007 * [sqrt(n%) - 2.5] / 7.5

Step count = 134.27 * sqrt(n%) - 335.67

It is also determined that the step count for a fully-opened iris is 1700. This would amount to 230%. The above equation satisfies aperture settings from 6.25% to 230%.

Attached to this article is an Excel spreadsheet that can be used to calculate the Aperture setting or Iris Size.

 

  • Was this article helpful?