How to set up iTEVA's IRINSE when using line switching
- iCAP 6500 Radial
- iCAP 6500 Dual
- iCAP 6300 Radial
- iCAP 6300 Dual
Intelligent rinse does in fact support line switching. Line switching takes place for every intelligent rinse sample. This might not be apparent as the results from the intelligent rinse samples aren't shown but that is indeed what is happening.
So when setting up the IRINSE table set, check table values for both lines – initially the high limit line will be used, however as the concentration falls the lower limit line will become the one used for the comparison. For example, if you want the session to proceed when the concentration has fallen below 1ppm then just set that in the IRINSE check for both lines.
Let's say for example we have 2 Na lines set up as follows:
|Concentration range||0-100 ppm||>100 ppm|
|IRINSE limit||0.1 ppm||1 ppm|
Now set up the IRINSE table with both Na lines in it, and make sure that they're both ticked so both will be used as part of the go/no-go criteria for rinsing.
Let's say we now run a sample at 500 ppm. Both lines will be used for the measurement but as the sample is greater than 100 ppm, only the Na818 line will report data.
The autosampler will now move to rinse and start washing.
An intelligent rinse is really just another sample measurement (but without printing the data), the wash is measured and maybe the concentration is still high so it will check against the limit for Na818, another measurement is taken and maybe it's still high until eventually the wash concentration falls below 100 ppm. Now it will check against the limit for the Na589 line.
Finally when the concentration falls below 0.1 ppm (in the example above) the autosampler will move on to the next sample.
One thing to be careful of is the delay time and the "max attempts" for the intelligent rinse. If the delay is short and the number of attempts is set quite low (e.g., 5) you may run out of attempts for a high concentration sample.
This means it will be checking against the limit for the 818 line, run out of attempts, fail the rinse and then move on without ever getting to use the limit set for Na 589.
It's hard to know exactly which values you should use because it will depend on the levels you're measuring, but start out with "10" and then keep an eye on it during a run and, if necessary, increase the number of attempts.