It has been noted that after collecting a set of well plate data the file is not saved correctly in the OMNIC secured folder.

Incorrect messages were shown by Array Automation software when used with the Raman software.

Raman auto calibration was being executed during the Array Automation collects. This has been turned off during the collection.

Some types of entries can cause error conditions that are difficult for the user to work around, This has been improved along with improvement in the calculation of the rotation of the array.

In some conditions the rotation calculation did not produce the correct result.

When the customer collects an array with the iS50 Raman, the array file is not saved in the protected folder, shown in Security Administration. Instead it is saved c:\my documents\OMNIC\array. No option to set the secure directory for Array Automation is available in the Security Administration program. In addition, Array data that is split from the Array measurements goes into the Array directory. In a secure system the user cannot normally navigate to the Array directory to open the split out spectra (*.spa files).

A problem where the instrument calibration can be initiated in between the measurements of an Array Automation run has been fixed.

When selecting a grid in each well for all wells, closing the array setup, and then re-entering the setup and selecting the methods tab the software crashes.

When collecting an array of samples, cancel the collection. Then start another collection and immediately cancel the collection again and it will crash.

When template calibration is being done on a DXR SmartRaman spectrometer with a well plate holder installed in the UPS accessory, a window is displayed showing spectra collected at the current position of the well plate holder.

On entry, the Move to Well dialog would occasionally indicate that it was at the wrong well.

When Array Automation is being used with an Almega spectrometer or a DXR *Raman Microscope, the Objective control in the Methods tab of Array Setup now displays the name of the objective whose calibration is selected in the Select Calibration dialog accessible from the System Configuration dialog in µView software or OMNIC Atlµs software. If this is incorrect, use the latter dialog to select the correct one. If OMNIC for Dispersive Raman 8.1 is being used, the Objective control in the Bench tab of Experiment Setup can also be used to specify the currently installed objective.

Because the new Autofocus before collect operation in OMNIC for Dispersive Raman is more flexible than the Spectrum Autofocus operation in Array Automation, if this is desired during Collect Array, it must be specified on the Advanced tab of Experiment Setup rather than in the Methods tab of Array Setup.

Multivariate Curve Resolution (MCR) has been added as a reanalysis method for Array data sets.

The Split Array function in the Array menu which allows Array data sets to be split into separate .spa files has been enhanced to allow any camera images saved with the data set to be saved in separate .bmp, .jpg or .tif format image files. The naming of the output files from Split Array has also been made more flexible.

A new command has been added to the Array menu which allows creation of Array data sets from individual .spa files and combination of Array data sets into a larger data set. The resulting .srs files can be opened and analyzed using the metrics available to the Reanalyze function.

The format of the LIMS command file has been expanded to allow individual spectra collected during Collect Array to be saved in separate .spa or .csv format files in addition to being added to the .srs file.

If the Require Signature When Saving Spectrum policy in Thermo Security Administration is enabled, signatures will be requested for all files saved by Array Automation. These include the .srs files saved by Collect Array, Create Array and Save Array As and the .spa files that are created by Split Array. Before this version it was necessary to use the Sign Files command to sign these files.

It is no longer necessary to remove the zscalefactor parameter from the OMNIC_mc.ini file.

Even if the "Disable delete, rename and right-click in file dialog boxes" feature of Thermo Security Administration is enabled, users are allowed to do these operations and change directories when saving Array Automation data.

Previously the Video autofocus option in the Methods tab of Array Setup was available only with the Prior stages used on Nicolet Almega systems. This option is now also available for the stages used on FT-Raman systems. With any stage, if this option is selected, a message box is displayed advising you that the current objective must be selected via the System Configuration dialog of µView or OMNIC Atlµs, and the objective must have been calibrated for Autofocus. Consult the µView or OMNIC Atlµs Help Topics for instructions.

When Array Automation is used with OMNIC for Almega, the latter’s new smart background and auto exposure options can be used for spectra collected by Collect Array. When auto exposure is used, no Approximate collection time is displayed in the Methods tab of Array Setup since there is no way of knowing what the collection time per spectrum will be.

When doing a Collect Array with Grid mode specified for one or more wells in the plate, there was an occasional skipping of grid positions leading to spectra being assigned to incorrect wells in the well display. (The titles of the spectra were correct.)

After doing a Cluster or Group reanalysis of an Array data set, if the similarity level is changed in the Cluster Analysis Results or Group Analysis Results dialog, the position of the Similarity scroll bar in the Open Data Set window display is now set accordingly.

If OMNIC Atlµs is installed on your system, when µView is run, a message box is shown saying that "OMNIC Atlµs is currently running. This may cause conflicts with video displays." This is to warn you that you cannot use µView and OMNIC Atlµs simultaneously to display the video camera image.

After installing Array Automation, the System Configuration command in µView program (MicroVw.exe) or OMNIC Atlµs must be used to make sure that the correct microscope and stage are selected and correctly configured. Consult the µView or OMNIC Atlµs Help Topics for instructions.

After upgrading these systems it is necessary to edit the OMNIC_mc.ini file residing in the directory C:/My Documents/OMNIC. Find the line zscalefactor=-10 and comment out this line by placing a semicolon <;> in front of it. Save this file, exit the editor and restart OMNIC. Failure to implement these steps will cause the z axis movement to reverse in the newly upgraded 7.3 relative to the movement in 7.2a.

When doing a kinetics collect using an Almega system, a new background was collected before each collection of spectra from the samples in the plate. The extra background collections have been eliminated.

Available from Thermo Fisher Scientific Customer Support is a holder for eight or twelve capillary tubes that can be used in place of a well plate. Templates for this holder have been added to the master template file. When one of these templates is selected for use in Array Setup, the sample positions in the Wells tab, in the Move to Well dialog, and in the Collect Array and Array view windows are represented by horizontal or vertical bars instead of circles or squares. Each bar corresponds to a capillary.

A button labeled "Save video images" has been added to the Methods tab of Array Setup. If it is checked, during a subsequent Collect Array operation, video camera images of the sample will be saved for each spectrum. These will be added to the .srs file which contains the sample spectra, and will be displayed when the spectra are examined later using Array: Open Data Set. NOTE: Each image adds approximately 0.5 Mbytes to the size of the .srs file.

The metrics available in the Methods tab of Array setup now include Peak height ratio and Peak area ratio. These metrics were previously only available for reanalysis of a data set.

The metrics available in the Methods tab of Array setup now include Correlation. To use this metric during a Collect Array, use the "Select Reference" button to choose the spectral file from disk that is to be used as the correlation reference. The spectral resolution of the selected file must match that selected for the data collection.

Normalize has been added to the functions available in Array: Apply Function.

In addition to using a spectrum from the data set as the reference for a Correlation reanalysis, it is now possible to use an external spectrum as the reference. The spectrum my be copied from an OMNIC window and pasted into the Array: Open Data Set window or the new Select Reference button may be used to open a spectrum file from disk and load it into the latter window.

Group analysis, Cluster analysis, and Principal component analysis are new metrics available for reanalysis of Array Automation data sets. The first two sort the spectra in the data set into groups of similar spectra. The last lets you find spectral variance in terms of its principal components. Consult the on-line Help for details about using these metrics.

If OMNIC DS is installed on the system, the Save Sequence dialog will contain a checkbox allowing sequence files to be signed. The Sign Files item in the Files menu can be used to sign Array Automation data sets after they have been collected.

A new Array menu item named Enable LIMS activates the software to accept instructions from a LIMS-generated file that specifies which wells in a plate are to be measured during a Collect Array operation and how the spectra are to be collected and analyzed. A description of how to use this feature in contained in the LIMS in AA.pdf file.

If regedit is use to specify a new string parameter named JCAMPDIrectory in the Registry in the HKEY_LOCAL_MACHINE/SOFTWARE/Nicolet Instrument/OMNIC/DLLs/ArrayDLL key, and if it defined as a valid (writable) directory on the system, all spectra collected during subsequent Collect Array operations will be saved in JCAMP-DX format in the defined directory, in addition to being saved in the Array data set (.srs) file. Each spectrum will be saved in a separate file whose name is derived from the data set title and the well from which it was obtained.