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Thermo Fisher Scientific

AN 50780 - Determination of Moisture Content in Freeze-Dried Materials by FT-NIR Spectroscopy


The feasibility of Fourier transform near-infrared spectroscopy (FT-NIR) for determination of moisture in lyophilized preparation of folinic acid calcium salt (Leucovorin Ca) was examined. FT-NIR spectra were collected by diffuse reflectance with the Integrating Sphere module of a Nicolet™ Antaris™ Method Development Sampling System from Thermo Electron Corporation. Stepwise multiple linear regression (SMLR) was used for calibration. The calibration results showed that FT-NIR spectroscopy is a suitable method for quantification of moisture in freeze-dried materials.


Antaris FT-NIR