Ghost peaks
Issue
How to troubleshoot if the unit is experiencing ghost peaks?
Environment
- Vanquish Flex Binary UHPLC System
- Vanquish Flex Quaternary UHPLC System
Resolution
| POSSIBLE CAUSE | REMEDIAL ACTION |
| Contamination (Typically injector or column) | Flush the system using an appropriate solvent. |
| Improper reference wavelength | The sample must not absorb in the range of the reference wavelength. Consider using a method without reference wavelength. |
| Selected bandwidth too high | Select a lower bandwidth. |
| Degassing channels contaminated | Purge the pump (all channels) to rinse the degassing channels (refer to Pump Operating Manual). |
For additional troubleshooting help try downloading the Thermo Scientific HPLC Troubleshooting Guide App. The app is an extensive tool to effectively diagnose various potential issues with HPLC analysis.
Thermo Scientific HPLC Troubleshooting Guide App (Apple App Store)
Thermo Scientific HPLC Troubleshooting Guide App (Google Play)