Selecting and installing cryopreservation equipment

Issue

How to:

• Differentiate various types of controlled rate freezing methods and their purposes
• Develop a protocol for freezing a selected sample in which the potential for cell viability is maximized
• Understand and identify sample phase changes
• Select an appropriate storage system and accessories to meet your required protocol, facility restraints, and budget
• Develop an installation and operational qualification (IQ/OQ) procedure required for a Good Manufacturing Practices (GMP) laboratory

Environment

Cryopreservation program or laboratory

Resolution

The attached Cryopreservation Guide is a practical tool for scientists who are responsible for an organization’s cryopreservation program. The guide presents the following:

• Overview of the cryobiology of cell and tissue preservation
• Comprehensive methodology for selecting and operating cryopreservation equipment
• Guidance regarding installation and operational qualifications, and safety required in a GMP laboratory