Troubleshooting Split Peaks in Chromatography

Issue

Users may observe split peaks in chromatography results, which can affect the accuracy and reliability of the analysis.

Check Sample Loop:
- Verify that all sample loops are of the same size.
- Ensure correct installation and functionality of the sample loops.
Inspect Sample Concentration:
- Determine the concentration of the samples being injected.
- Ensure the concentration is within the acceptable range for the chromatography system.
Examine Peak Size:
- Assess the size of the peaks being observed.
- Compare with expected peak sizes based on sample concentration and system settings.
Test Different Sample Sizes:
- Dilute or reduce the sample size and observe if the peak shapes improve.
- If peak shapes improve, it may indicate column overloading.
Check Column Condition:
- Inspect the condition of the chromatography column.
- Replace the column if it shows signs of wear or contamination.
Analyze Suppressors:
- Check for contaminated or leaking suppressors.
- Replace suppressors if necessary.
Examine Water Source:
- Verify the quality of the water used in the system.
- Use a different source of water if contamination is suspected.
Consult Technical Support:
- If the issue persists, contact technical support for further assistance.
- Provide details of the steps taken and observations made during troubleshooting.

Split peaks in chromatography can be caused by: