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Thermo Fisher Scientific

TurboFlow Column Wash Solvent

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Issue

How do I prepare the TurboFlow Column wash solvent?

Environment

  • LX-2
  • LX-4

Resolution

Prepare a 45/45/10 acetonitrile/isopropanol/acetone solution in a clean 2-liter bottle by mixing the following:

• 900 mL of LC/MS-grade acetonitrile

• 900 mL of LC/MS-grade isopropanol

• 200 mL of LC/MS-grade acetone

 

NOTE:

  • Use this solution to fill the column wash solvent reservoir.
  • The solution is stable for 30 days at room temperature. 
  • You can use column wash solvent as the autosampler's organic wash solution when carryover is an issue.