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- https://knowledge1.thermofisher.com/Chromatography/Liquid_Chromatography/Aria_and_Transcend_Systems/Transcend_Knowledge/How_to_create_a_reference_for_tracking_injectionsSelect the system component that you want to track in the upper portion of the Maintenance dialog box. To have the system notify you when a specific number of injections that involve the selected comp...Select the system component that you want to track in the upper portion of the Maintenance dialog box. To have the system notify you when a specific number of injections that involve the selected component has elapsed, type the number of injections. To not have the system notify you when a number of injections have elapsed using the component, leave the box value set to 0. With this check box selected, an injection on any of the channels updates the count for this reference.
- https://knowledge1.thermofisher.com/Chromatography/Liquid_Chromatography/Aria_and_Transcend_Systems/Transcend_Knowledge/Replacing_the_valve_needle_sealIssue How do I replace the needle seal? Environment LX-2 LX-4 Resolution Unscrew the needle guide from the valve. NOTE: The Vanquish Dual Split Sampler injectors do not use valve needle seals.
- https://knowledge1.thermofisher.com/Chromatography/Liquid_Chromatography/Aria_and_Transcend_Systems/Transcend_Knowledge/TurboFlow_Column_Wash_SolventIssue How do I prepare the TurboFlow Column wash solvent? Prepare a 45/45/10 acetonitrile/isopropanol/acetone solution in a clean 2-liter bottle by mixing the following: • 900 mL of LC/MS-grade aceton...Issue How do I prepare the TurboFlow Column wash solvent? Prepare a 45/45/10 acetonitrile/isopropanol/acetone solution in a clean 2-liter bottle by mixing the following: • 900 mL of LC/MS-grade acetonitrile • 900 mL of LC/MS-grade isopropanol • 200 mL of LC/MS-grade acetone Use this solution to fill the column wash solvent reservoir. The solution is stable for 30 days at room temperature. You can use column wash solvent as the autosampler's organic wash solution when carryover is an issue.
- https://knowledge1.thermofisher.com/Chromatography/Liquid_Chromatography/Aria_and_Transcend_Systems/Transcend_Knowledge/Replacing_the_Analytical_Column_every_2000_injections(TCC module) Click the On button so that the On/Off indicator in the upper-left of the right pane turns dark green and the On button changes to the Off button. Loosen the knob on one end of the column...(TCC module) Click the On button so that the On/Off indicator in the upper-left of the right pane turns dark green and the On button changes to the Off button. Loosen the knob on one end of the column until the fitting disengages from the column. Loosen the fitting on the other end of the column while holding onto the column. Remove the end cap from one end of the new column and insert the column fitting. Remove the end cap from the other end of the column and insert the column fitting.
- https://knowledge1.thermofisher.com/Chromatography/Liquid_Chromatography/Aria_and_Transcend_Systems/Transcend_Knowledge/Preparation_of_Mobile_Phase_SolventsNOTE: If caps are not available, use aluminum foil to secure the solvent lines in the bottle and protect the solvent from dust. Ensure that the mobile phase line with attached solvent filter reaches t...NOTE: If caps are not available, use aluminum foil to secure the solvent lines in the bottle and protect the solvent from dust. Ensure that the mobile phase line with attached solvent filter reaches the bottom of its intended reservoir. Before each batch, vigorously swirl the mobile phase bottles and look for particulates that might be floating or moving in the liquid. Replace the solvent filters and purge the lines fully with new, clean, LC/MS-grade mobile phase.
- https://knowledge1.thermofisher.com/Chromatography/Liquid_Chromatography/Aria_and_Transcend_Systems/Transcend_Operator_Manuals/System_plumbing_guideDescription: This article provides a guide for the plumbing specifications for the various system types. Environment: Transcend II: TLX1 TLX2 TLX4 Attachment(s): File Last Modified Rev D - System plum...Description: This article provides a guide for the plumbing specifications for the various system types. Environment: Transcend II: TLX1 TLX2 TLX4 Attachment(s): File Last Modified Rev D - System plumbing guide.pdf November 30, 2021
- https://knowledge1.thermofisher.com/Chromatography/Liquid_Chromatography/Aria_and_Transcend_Systems/Transcend_Knowledge/Replacing_the_Rotor_SealsThe following table lists the appropriate rotor seal type for each valve type. Use a 9/64 Allen wrench to loosen the two screws on the face of the valve. Lift the rotor seal by lifting one of the tabs...The following table lists the appropriate rotor seal type for each valve type. Use a 9/64 Allen wrench to loosen the two screws on the face of the valve. Lift the rotor seal by lifting one of the tabs. Line up the new rotor seal tabs to the slots on the valve and with the rotor seal grooves facing toward you. The slots are aligned so that the seal can only be installed at the proper orientation. Reinstall the valve face so that ports 6 and 1 appear at the top.
- https://knowledge1.thermofisher.com/Chromatography/Liquid_Chromatography/Aria_and_Transcend_Systems/Transcend_Knowledge/Replacing_the_TurboFlow_Column_every_300-1000_injectionsHow do I replace the TurboFlow column? Turn off the column heater and allow the column heater to cool to room temperature. Click a column heater that appears under the appropriate channel name. To tur...How do I replace the TurboFlow column? Turn off the column heater and allow the column heater to cool to room temperature. Click a column heater that appears under the appropriate channel name. To turn off the column heater, click the Enable button so that it shows dark green. Repeat step b and step c to turn off the second column heater. When the column heater has cooled, unwrap the column heater from the column. Note: Use only your fingers to manipulate the tubing fittings on Prelude systems.
- https://knowledge1.thermofisher.com/Chromatography/Liquid_Chromatography/Aria_and_Transcend_Systems/Transcend_Knowledge/Version__1.0_-_Cleaning_the_solvent_lines%2C_pumps%2C_and_valvesRemove all the lines from the mobile phase bottles, place them into LC/MS grade deionized water, and do the following: Then, load a vial of water into the appropriate position on the autosampler and p...Remove all the lines from the mobile phase bottles, place them into LC/MS grade deionized water, and do the following: Then, load a vial of water into the appropriate position on the autosampler and perform the run. Load a vial of water into the appropriate position on the autosampler and perform the run. If you use buffers with high salt concentrations, this article provides the steps to cleans the lines, pumps, and valves, and helps prevent a salt buildup.
- https://knowledge1.thermofisher.com/Chromatography/Liquid_Chromatography/Aria_and_Transcend_Systems/Transcend_Operator_Manuals/Man-LC-Transcend-Systems-Operation-DOC4822.1201-EN-ADescription: This manual describes the functional features and operating principle of the Transcend systems, and provides instructions for installation, start up, shut down, operation, maintenance and...Description: This manual describes the functional features and operating principle of the Transcend systems, and provides instructions for installation, start up, shut down, operation, maintenance and troubleshooting of your system. Environment: Vanquish Transcend™ Multichannel systems Attachment(s): File Last Modified Man-LC-Transcend-Systems-Operation-DOC4822.1201-EN-A.pdf March 11, 2025