Peak Area Precision
Issue
How to troubleshoot if the unit is experiencing peak area precision?
Environment
- Vanquish Flex Binary UHPLC System
- Vanquish Flex Quaternary UHPLC System
Resolution
POSSIBLE CAUSE | REMEDIAL ACTION |
Contamination in the system | Flush the system using an appropriate solvent. |
Autosampler carry-over | The needle may be contaminated. Wash the needle in the wash port. If carry-over persists, replace the needle seat (refer to Split Sampler Operating Manual). |
Unstable environmental conditions |
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Capillary connections not installed properly or not tight, dead volumes in capillary connections |
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For additional troubleshooting help try downloading the Thermo Scientific HPLC Troubleshooting Guide App. The app is an extensive tool to effectively diagnose various potential issues with HPLC analysis.
Thermo Scientific HPLC Troubleshooting Guide App (Apple App Store)
Thermo Scientific HPLC Troubleshooting Guide App (Google Play)