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Thermo Fisher Scientific

Peak Area Precision


How to troubleshoot if the unit is experiencing peak area precision?


  • Vanquish Flex Binary UHPLC System
  • Vanquish Flex Quaternary UHPLC System


Contamination in the system Flush the system using an appropriate solvent.
Autosampler carry-over The needle may be contaminated. Wash the needle in the wash port. If carry-over persists, replace the needle seat (refer to Split Sampler Operating Manual).
Unstable environmental conditions
  • Make sure that the temperature and air humidity are constant.
  • Use column thermostatting.
  • Avoid draft.
  • Use insulation on the capillary from the column compartment to the detector.
Capillary connections not installed properly or not tight, dead volumes in capillary connections
  • Properly install the capillary connections.
  • Tighten the capillary connections. Replace capillary.

For additional troubleshooting help try downloading the Thermo Scientific HPLC Troubleshooting Guide App. The app is an extensive tool to effectively diagnose various potential issues with HPLC analysis.

Thermo Scientific HPLC Troubleshooting Guide App (Apple App Store)

Thermo Scientific HPLC Troubleshooting Guide App (Google Play) 


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