SOLAAR Software Calibration Data

Issue

The calibration data shown within Solaar such as the correlation coefficient can be drastically different depending on which calibration fitting is use.

Environment

• Solaar

Resolution

To change the calibration curve, right click on a calibration standard or sample and choose "Change Line Fit".  The "Linear Least Squared" will show the correlation coefficient as the "Fit" on the calibration curve.  If the "Segmented Curve" is used there will not be a correlation coefficient because the segmented curve is a calibration curve that is split into multiple calibration lines rather than a linear least squares curve that is a single line that is fit using linear least squares.  The segmented calibration using only two points per curve, so the correlation coefficient would always be 1.